Annoying Nasus QsL issue that has existed forever
The issue is that you cannot cancel Q-empowered autoattacks once the animation has started, the way you can cancel normal autoattacks. So if you press Q and mistakenly click on the wrong minion or the enemy laner, you can't correct yourself and you lose the stack.
In fact, Q-empowered autos can't be interrupted by any means. Stun Nasus, knock him out of range, it doesn't matter, as long as the animation has started the Q damage is going to be applied. So this is an issue both when playing Nasus and playing against Nasus.