I don't have to try it, it's mathematically impossible for you to have Vayne-style burst with an on-hit dps build.
I can even numerically outline exactly how that's bullshit.
Math time!
You are two-shotting a Vayne. She has no health items. A Level 18 Vayne has 1909 health. Assume no resistances. Teemo now has to deal 1909 damage with a Q and an auto-attack.
Level 18 Teemo with your specifications has:
101 base AD
AS irrelevant, you're claiming Q-AA kills squishies
10.8 AD (Runes)
35.28 AP (Runes)
18-12-0 mastery set-up, relevant damage masteries being:
Double-Edged Sword - Dealing 5% increased damage
Natural Talent - 10 AD and 15 AP at Level 18
Since you're two-shotting, Fervor (which is literally shittier on on-hit now) and Piercing Thoughts (7% magic pen) shouldn't be relevant - you're gonna two-shot a squishy. I'm even gonna assuming you're fighting someone with ZERO resistances to be fair.
- 4% max hp as physical damage
- 25 AD, 6% current hp on-hit, 10% max hp active
- 80 AP, 15 (+15% AP) on-hit magic damage
- Crit Chance, 15 on-hit physical damage
Boots don't matter, I'm assuming no resistances
You give no 6th item, I assume Guinsoo's
- 35 AD, 50 AP, 15 on-hit magic damage, stacks don't matter since you insist on a two-shot
IN SUMMARY, Teemo has stats totalling (approximately):
182 AD
180 AP
Calculating Double-edged sword, damaging effects and abilities deal a 1909 health Vayne:
Q - Blinding Dart: 260 (+80% AP) + 0.05(260 x 80% AP) = 424 magic damage
- 10% max hp active - assuming proper order, it deals 201 physical damage)
Auto-attack deals:
382 flat basic attack physical damage (I'm even assuming this auto crit)
- 4% max hp on-hit (80 physical damage)
- 6% current hp on-hit (currently, 81 physical damage)
- 15 (+15% AP) on-hit (44 magic damage)
- 15 on-hit (boosted to 16 physical damage)
- ^See Above for magic damage^
Toxic Dart - 50 (+30% AP) + Mastery boost = 109 magic damage
Math dictates:
424 + 201 + 382 + 80 + 81 + 44 + 16 + 16 = 1353 total damage
Last I did math, 1909 damage needed > 1353 damage dealt. By 556.
As Teemo, you need to get TWO (count 'em, TWO) more consecutive CRITICAL auto-attacks to kill Vayne. With 30% critical strike chance. You're not two-shotting shit.
I was even being nice by not including Vayne's resistances. Add Vayne's basic resistances (76.8 base armor, 30 magic resist), and the physical damage is cut by about 45%, with the magic damage taking a 25% reduction. You're not even killing the Vayne with 4 critical auto-attacks in a row. Your ass is getting condemned into a 1100 Q Critical with a 200 damage Shiv Critical Proc and death ensues.
I'm not going to waste my time trying to get into a jungle Teemo game just to fail at being an assassin with the LEAST assassiny damage build possible.